RDB 2005 Factor V / II
A prevalence of about 1:20 for heterozygous genotype makes factor V-Leiden the most widespread genetic risk factor for deep venous thrombosis in general public. Reason is a SNP in factor V gene which makes the protein resistant against inactivation. Insufficient inactivation of factor V means a higher risk for thrombosis.
Very similar effect is caused by a mutation in regulatory region of prothrombin gene. Here the mutation causes higher synthesis of factor II resulting in more thrombin and therefor higher risk for thrombosis.
AID Factor V/II Kit enables detection of thrombosis risk alleles.
- Detection of risk allele FV:Q506 in Factor V gene and G20210A in Factor II gene
- Differentiation between homozygous and heterozygous genotype
- PCR with subsequent lineprobe assay
- Specimen: human DNA isolated from buccal swab, Citrate- or EDTA-blood or biopsy
- Control bands on every strip show correct DNA isolation, amplification and hybridization
- Results within 4 hours
- Suited for automated systems
- Evaluation and documentation with AID Scanning system